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Catalog Number Size Price
LS-C745487-50 50 µg (0.93 mg/ml) $567 
HIST3H3 Antibody - Immunofluorescence of Histone H3 K27me3 Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 K27me3 antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 K27me3 is nuclear and chromosomal. Staining: Histone H3 K27me3 is expressed green and the nuclei are counterstained blue with DAPI.
HIST3H3 Antibody - Western Blot of Histone H3 K27me3 Antibody. Western Blot analysis against untreated cell extracts. Lane 1: Molecular Weight Marker. Lane 2: HeLa cell lysates. Lane 3: 3T3 cell lysates. Lane 4: PC12 cell lysates. Lane 5: A431 cell lysate. Primary antibody: Histone H3 K27me3 antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: MB-073 BlockOut overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 K27Me3. Other band(s): none.
HIST3H3 Antibody - Dot Blot of Histone H3 K27 Me3 Antibody. Lane 1: Non Modified Peptide. Lane 2: Histone H3 K27-Me1 Peptide. Lane 3: Histone H3 K27-Me2 Peptide. Lane 4: Histone H3 K27-Me3 Peptide. Lane 5: Histone H3 K27-Ac Peptide. Dilution: 0.1µg/mL. Blocking: 5% Blotto at RT 30 min. Exposure time: 13 sec.
HIST3H3 Antibody - Immunofluorescence of Histone H3 K27me3 Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 K27me3 antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 K27me3 is nuclear and chromosomal. Staining: Histone H3 K27me3 is expressed green and the nuclei are counterstained blue with DAPI.
HIST3H3 Antibody - Western Blot of Histone H3 K27me3 Antibody. Western Blot analysis against untreated cell extracts. Lane 1: Molecular Weight Marker. Lane 2: HeLa cell lysates. Lane 3: 3T3 cell lysates. Lane 4: PC12 cell lysates. Lane 5: A431 cell lysate. Primary antibody: Histone H3 K27me3 antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: MB-073 BlockOut overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 K27Me3. Other band(s): none.
HIST3H3 Antibody - Dot Blot of Histone H3 K27 Me3 Antibody. Lane 1: Non Modified Peptide. Lane 2: Histone H3 K27-Me1 Peptide. Lane 3: Histone H3 K27-Me2 Peptide. Lane 4: Histone H3 K27-Me3 Peptide. Lane 5: Histone H3 K27-Ac Peptide. Dilution: 0.1µg/mL. Blocking: 5% Blotto at RT 30 min. Exposure time: 13 sec.
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Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Me3‑Lys27, IF, WB) LS‑C745487

Polyclonal Rabbit anti‑Human HIST3H3 Antibody (Me3‑Lys27, IF, WB) LS‑C745487

Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Me3-Lys27) Antibody
Application:
IF, WB, IP, ELISA, DB
Reactivity:
Human, Mouse, Rat, C. elegans
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C745487-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIST3H3 Rabbit anti-Human Polyclonal (Me3-Lys27) Antibody
Application:
IF, WB, IP, ELISA, DB
Reactivity:
Human, Mouse, Rat, C. elegans
Format:
Unconjugated, Unmodified

Specifications

Description
HIST3H3 antibody LS-C745487 is an unconjugated rabbit polyclonal antibody to HIST3H3 (Me3-Lys27) from human. It is reactive with human, mouse, rat and other species. Validated for DB, ELISA, IF, IP and WB.
Target
Human HIST3H3
Synonyms
HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
Host
Rabbit
Reactivity
Human, Mouse, Rat, C. elegans (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, C. elegans (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.
Epitope
Me3-Lys27
Specificity
A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000)
  • Dot Blot
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST3H3
Q16695 NM_003493 NP_003484.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/26/2024