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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745500-50 | 50 µg (0.21 mg/ml) | $582 |
![HIST3H3 Antibody - Western Blot of Histone H3 pT3/R2Me2s (RABBIT) Antibody. Western Blot analysis against untreated cell extracts, 5 minute exposure. Lane 1: HeLa cell lysates. Lane 2: NIH/3T3 cell lysates. Lane 3: Cos 7 cell lysates. Load: 35 µg per lane. Primary antibody: Histone H3 pT3/R2Me2s antibody at 1µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 [pT3/R2Me2s]. Other band(s): non-specific.](https://lsbio-7d62.kxcdn.com/image2/hist3h3-antibody-ls-c745500/446127_3552866.jpg)
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Polyclonal Rabbit anti‑Human HIST3H3 Antibody (IF, WB) LS‑C745500
Polyclonal Rabbit anti‑Human HIST3H3 Antibody (IF, WB) LS‑C745500
Antibody:
HIST3H3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HIST3H3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
HIST3H3 antibody LS-C745500 is an unconjugated rabbit polyclonal antibody to human HIST3H3. Validated for DB, IF and WB.
Target
Human HIST3H3
Synonyms
HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Histone H3 pT3/R2Me2s affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the pT3 and R2Me2s site of human Histone H3.
Specificity
This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Applications
- Immunofluorescence (1:50 - 1:100)
- Western blot (1 µg/ml)
- Dot Blot
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIST3H3
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Featured Products
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Source:
Human
Tag:
Myc-DDK (Flag)
Reactivity:
Human
Range:
31.25-2000 pg/ml
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/10/2024
Date Requested: 5/10/2024
