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Antibodies > HIST1H3A > LS-C676830
 
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Catalog Number Size Price
LS-C676830-50 50 µl $298 
LS-C676830-100 100 µl $370 
HIST1H3A Antibody - Immunocytochemistry analysis diluted at 1:50 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HIST1H3A Antibody - Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) diluted at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H3A Antibody - Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H3A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
HIST1H3A Antibody - Western Blot Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate(all treated with 30mM sodium butyrate for 4h) All Lanes: HIST1H3A antibody at 1.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 KDa Observed band size: 16 KDa
HIST1H3A Antibody - Chromatin Immunoprecipitation Hela (10E6, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (Butyrly-HIST1H3A (K9) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
HIST1H3A Antibody - Immunocytochemistry analysis diluted at 1:50 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HIST1H3A Antibody - Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) diluted at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H3A Antibody - Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H3A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
HIST1H3A Antibody - Western Blot Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate(all treated with 30mM sodium butyrate for 4h) All Lanes: HIST1H3A antibody at 1.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 KDa Observed band size: 16 KDa
HIST1H3A Antibody - Chromatin Immunoprecipitation Hela (10E6, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (Butyrly-HIST1H3A (K9) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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Polyclonal Rabbit anti‑Human HIST1H3A Antibody (IHC, IF, WB) LS‑C676830

Polyclonal Rabbit anti‑Human HIST1H3A Antibody (IHC, IF, WB) LS‑C676830

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Antibody:
HIST1H3A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$298
LS-C676830-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HIST1H3A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H3A antibody LS-C676830 is an unconjugated rabbit polyclonal antibody to human HIST1H3A. Validated for ELISA, ICC, IF, IHC and WB.
Target
Human HIST1H3A
Synonyms
HIST1H3A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Peptide sequence around site of butyrly-Lys (9) derived from human Histone H3
Applications
  • IHC
  • ICC
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H3A
NP_003520.1

Publications (0)

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Featured Products

HIST1H3A Antibody - Detection of Human and Mouse Histone H3 (S10) by Immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 Detection: DAB.
HIST1H3A Antibody (phospho‑Ser10) LS‑C288638
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry
HIST1H3A Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HIST1H3A Antibody LS‑C675322
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
HIST1H3A Antibody - Immunohistochemistry image of paraffin-embedded human lung cancer at a dilution of 1:100
HIST1H3A Antibody LS‑C674739
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Chromatin Immunoprecipitation
HIST1H3A Antibody - Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HIST1H3A Antibody LS‑C676951
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
HIST1H3A Antibody - Immunofluorescent analysis of Hela cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
HIST1H3A Antibody LS‑C674777
Species: Human
Applications: Immunofluorescence, ELISA
His Tag ELISA Kit
His Tag (Competitive EIA) ELISA Kit - LS-F55748
Reactivity: All species
Range: 24.69-2000 pg/ml
See all HIST1H3A Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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