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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIST1H2BB Antibody LS‑C675313 Best Seller
HIST1H2BB antibody LS-C675313 is an unconjugated rabbit monoclonal antibody to human HIST1H2BB. Validated for ELISA, Flow, ICC, IF and WB.
Catalog
Size
Price
LS-C675313-50
50 µl
$315
LS-C675313-100
100 µl
$425
Description
HIST1H2BB antibody LS-C675313 is an unconjugated rabbit monoclonal antibody to human HIST1H2BB. Validated for ELISA, Flow, ICC, IF and WB.
Target
Human HIST1H2BB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
affinity chromatography
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Immunogen
HIST1H2BB antibody was raised against a synthesized peptide
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About HIST1H2BB
P33778 NM_021062 NP_066406.1

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Immunocytochemistry

Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa

Immunocytochemistry

Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa

Immunocytochemistry

Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa

Immunocytochemistry

Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) diluted at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All Lanes:Acetyl-Histone H2B type 1-B (K20) antibody at 0.135µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa

Requested From: United States
Date Requested: 11/19/2018
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