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Catalog Number Size Price
LS-C674870-50 50 µl $298 
LS-C674870-100 100 µl $370 
HIST1H2AI Antibody - Immunocytochemistry analysis of Crotonyl-HIST1H2AG (K118) Antibody diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H2AI Antibody - Immunofluorescence staining of hela at a dilution of 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H2AI Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate (treated by 30mM sodium crotonylate for 4h) All lanes: HIST1H2AG antibody at 0.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
HIST1H2AI Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (Crotonyl-HIST1H2AG (K118) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
HIST1H2AI Antibody - Immunocytochemistry analysis of Crotonyl-HIST1H2AG (K118) Antibody diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H2AI Antibody - Immunofluorescence staining of hela at a dilution of 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
HIST1H2AI Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate (treated by 30mM sodium crotonylate for 4h) All lanes: HIST1H2AG antibody at 0.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
HIST1H2AI Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (Crotonyl-HIST1H2AG (K118) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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Polyclonal Rabbit anti‑Human HIST1H2AI Antibody (IF) LS‑C674870

Polyclonal Rabbit anti‑Human HIST1H2AI Antibody (IF) LS‑C674870

Antibody:
HIST1H2AI Rabbit anti-Human Polyclonal Antibody
Application:
IF, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$298
LS-C674870-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIST1H2AI Rabbit anti-Human Polyclonal Antibody
Application:
IF, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H2AI antibody LS-C674870 is an unconjugated rabbit polyclonal antibody to human HIST1H2AI. Validated for ELISA and IF.
Target
Human HIST1H2AI
Synonyms
HIST1H2AI
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Peptide sequence around site of crotonyl-Lys (118) derived from human Histone H2A type 1.
Applications
  • Immunofluorescence
  • ELISA
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H2AI
NP_003500.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/23/2024