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Catalog Number Size Price
LS-C804397-100 100 µl (1 mg/ml) $379 
LS-C804397-200 200 µl (1 mg/ml) $421 
HIST1H1E Antibody - 1:100 staining mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HIST1H1E Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HIST1H1E Antibody - Western blot analysis of Phospho-Histone H1.3 (T17) +H1.4 (T17) using 293 whole cells lysates
HIST1H1E Antibody - 1:100 staining mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HIST1H1E Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HIST1H1E Antibody - Western blot analysis of Phospho-Histone H1.3 (T17) +H1.4 (T17) using 293 whole cells lysates
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Polyclonal Rabbit anti‑Human HIST1H1E Antibody (phospho‑Thr17, IHC, IF, WB) LS‑C804397

Polyclonal Rabbit anti‑Human HIST1H1E Antibody (phospho‑Thr17, IHC, IF, WB) LS‑C804397

Antibody:
HIST1H1E Rabbit anti-Human Polyclonal (pThr17) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804397-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIST1H1E Rabbit anti-Human Polyclonal (pThr17) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H1E antibody LS-C804397 is an unconjugated rabbit polyclonal antibody to HIST1H1E (pThr17) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human HIST1H1E
Synonyms
HIST1H1E | H1.4 | Histone cluster 1, H1e | Histone H1b | H1 histone family, member 4 | H1s-4 | Histone 1 family 4 | Histone 1, H1e | Histone H1.4 | H1E
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Phospho-Histone H1.3 (T17) +H1.4 (T17).
Epitope
pThr17
Specificity
Phospho-Histone H1.3 (T17) +H1.4 (T17) Antibody detects endogenous levels of Histone H1.3 only when phosphorylated at Thr17.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H1E
P10412 NM_005321 NP_005312.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/1/2024