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Catalog Number Size Price
LS-C676835-50 50 µl $298 
LS-C676835-100 100 µl $370 
HIST1H1C Antibody - Immunocytochemistry analysis of Crotonyl-HIST1H1C (K158) Antibody diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H1C Antibody - Immunofluorescent analysis of Hela cells (treated with 30mM crotonylate for 4h) diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
HIST1H1C Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1c (Crotonyl-HIST1H1C (K158) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
HIST1H1C Antibody - Immunocytochemistry analysis of Crotonyl-HIST1H1C (K158) Antibody diluted at 1:50 and staining in Hela cells (treated with 30mM sodium crotonylate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HIST1H1C Antibody - Immunofluorescent analysis of Hela cells (treated with 30mM crotonylate for 4h) diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
HIST1H1C Antibody - Chromatin Immunoprecipitation Hela (4*10E6, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1c (Crotonyl-HIST1H1C (K158) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ß-Globin promoter.
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Polyclonal Rabbit anti‑Human HIST1H1C Antibody (IF) LS‑C676835

Polyclonal Rabbit anti‑Human HIST1H1C Antibody (IF) LS‑C676835

Antibody:
HIST1H1C Rabbit anti-Human Polyclonal Antibody
Application:
IF, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$298
LS-C676835-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIST1H1C Rabbit anti-Human Polyclonal Antibody
Application:
IF, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HIST1H1C antibody LS-C676835 is an unconjugated rabbit polyclonal antibody to human HIST1H1C. Validated for ChrIP, ELISA and IF.
Target
Human HIST1H1C
Synonyms
HIST1H1C | H1C | Histone cluster 1, H1c | Histone H1d | H1 histone family, member 2 | H1s-1 | Histone 1, H1c | H1.2 | Histone H1.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Peptide sequence around site of crotonyl-Lys (158) derived from human Histone H1.2
Applications
  • Immunofluorescence
  • ELISA
  • Chromatin Immunoprecipitation
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST1H1C
P16403 NM_005319 NP_005310.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024