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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HINT / HINT1 Antibody (Internal) IHC‑plus™ LS‑B4989
Note: This antibody replaces LS-C83931
HINT1 antibody LS-B4989 is an unconjugated rabbit polyclonal antibody to HINT1 (HINT) from human, mouse and rat. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4989-50
50 µg (1 mg/ml)
$425
Description
HINT1 antibody LS-B4989 is an unconjugated rabbit polyclonal antibody to HINT1 (HINT) from human, mouse and rat. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HINT / HINT1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30579 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B4989. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Usage
HINT1 antibody can be used for detection of HINT1 by Western blot at 1-2 ug/ml
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
About HINT / HINT1
P49773 NM_005340 NP_005331.1

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Images

Immunohistochemistry

Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.

Western blot

Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.

Western blot

Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.

Western blot

Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.

Western blot

Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.

Requested From: United States
Date Requested: 11/20/2018
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