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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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HINT / HINT1 Antibody IHC-plus™ LS-B4989

Note: This antibody replaces LS-C83931
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
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HINT / HINT1 Antibody IHC-plus™ LS-B4989

Note: This antibody replaces LS-C83931
Antibody:
Rabbit Polyclonal to Human HINT / HINT1
Application:
IHC, IHC-P, ICC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B4989-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HINT / HINT1
Application:
IHC, IHC-P, ICC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HINT1 antibody LS-B4989 is an unconjugated rabbit polyclonal antibody to HINT1 (HINT) from human, mouse and rat. Validated for ELISA, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human HINT / HINT1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HINT / HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1. The immunogen is located within the last 50 amino acids of HINT1.
Blocking Peptide
LS-E30579 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B4989. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HINT / HINT1
P49773 NM_005340 NP_005331.1

Validation

Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Reactivity: Rat
Range: 0.188-12 ng/ml
Flow cytometry of CD30 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Reactivity: Mouse
Range: 0.312-20 ng/ml
Reactivity:
Range: 3.12-200 µmol/l

Requested From: United States
Date Requested: 5/25/2019
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