Reactivity
Yeast
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Hexokinase [Yeast].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species is expected based on sequence homology.
Applications
- Western blot (1:500 - 1:1000)
- ELISA (1:1000 - 1:2500)
Usage
Anti-HEXOKINASE (RABBIT) Antibody can be used in applications such as Immunohistochemistry (IHC), Western blot (WB) Immunofluorescence (IF) ELISA. Specific conditions should be optimized by researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee