Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C745294-25 25 µl (1 mg/ml) $275 
HDAC1 Antibody - Immunohistochemistry of rabbit Anti-HDAC-1 Antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 as brown color indicates presence of protein, blue color shows cell nuclei.
HDAC1 Antibody - Immunohistochemistry of rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain.
HDAC1 Antibody - Immunofluorescence Microscopy of rabbit anti-HDAC1 Antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum. Primary antibody: HDAC1 antibody at 04 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 0.2 µg/mL for 45 min at RT. Localization: HDAC1 is nuclear. Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.
HDAC1 Antibody - Immunofluorescence Microscopy of rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 was used with Atto 425 (shown in red). Anti-Keratin monoclonal antibody was used with Dylight 488 (shown in green) to detect Keratin. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems).
HDAC1 Antibody - Western Blot of rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55kDa for HDAC-1. Other band(s): none.
HDAC1 Antibody - Western Blot of rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.
HDAC1 Antibody - Immunohistochemistry of rabbit Anti-HDAC-1 Antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 as brown color indicates presence of protein, blue color shows cell nuclei.
HDAC1 Antibody - Immunohistochemistry of rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain.
HDAC1 Antibody - Immunofluorescence Microscopy of rabbit anti-HDAC1 Antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum. Primary antibody: HDAC1 antibody at 04 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 0.2 µg/mL for 45 min at RT. Localization: HDAC1 is nuclear. Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.
HDAC1 Antibody - Immunofluorescence Microscopy of rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 was used with Atto 425 (shown in red). Anti-Keratin monoclonal antibody was used with Dylight 488 (shown in green) to detect Keratin. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems).
HDAC1 Antibody - Western Blot of rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55kDa for HDAC-1. Other band(s): none.
HDAC1 Antibody - Western Blot of rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.
1 of 6
2 of 6
3 of 6
4 of 6
5 of 6
6 of 6

Polyclonal Rabbit anti‑Human HDAC1 Antibody (aa450‑482, IHC, IF, WB) LS‑C745294

Polyclonal Rabbit anti‑Human HDAC1 Antibody (aa450‑482, IHC, IF, WB) LS‑C745294

Antibody:
HDAC1 Rabbit anti-Human Polyclonal (aa450-482) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745294-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HDAC1 Rabbit anti-Human Polyclonal (aa450-482) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC1 antibody LS-C745294 is an unconjugated rabbit polyclonal antibody to human HDAC1 (aa450-482). Validated for ELISA, IF, IHC and WB.
Target
Human HDAC1
Synonyms
HDAC1 | Histone deacetylase 1 | GON-10 | HD1 | RPD3 | RPD3L1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
Epitope
aa450-482
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.
Applications
  • IHC (1:200 - 1:1000)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC1
Q13547 NM_004964 NP_004955.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021