Human HCRT / Orexin

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH.

Human orexin A, an excitatory neuropeptide linked to alertness and wakefulness in humans.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• ELISA

Immunohistochemistry: LS-B31 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B31 was determined to be 10 mg/ml. Histology Positive Control Tissue: Hypothalamus.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O43612 NCBI: NM_001524 NP_001515.1