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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HBEGF / HB EGF Antibody (N‑Terminus, HRP) LS‑C447421
HB EGF antibody LS-C447421 is an HRP-conjugated rabbit polyclonal antibody to HB EGF (HBEGF) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C447421-100
100 µl (0.65 mg/ml)
$385

Popular HBEGF / HB EGF Products

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Western blot
HBEGF Antibody western blot of K562 cell line lysates (35 ug/lane). The HBEGF antibody detected the HBEGF protein (arrow).
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Western blot of recombinant HBEGF.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Antibody
Species: Mouse
Applications: ICC, Western blot

Product Description

HB EGF antibody LS-C447421 is an HRP-conjugated rabbit polyclonal antibody to HB EGF (HBEGF) from human, mouse, rat and other species. Validated for IHC and WB.

Specifications

Target
Human HBEGF / HB EGF
Synonyms
HBEGF, Dtr, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Gibbon, Mouse, Rat, Bat, Bovine, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HBEGF / HB EGF antibody was raised against synthetic peptide from N-Terminus of human HBEGF (Q99075, NP_001936). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Panda, Bovine, Bat, Pig (100%); Monkey, Galago, Marmoset, Elephant (92%); Dog (85%); Opossum (84%).
Epitope
N-Terminus
Specificity
Human HBEGF / HB EGF
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About HBEGF / HB EGF
Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. Q99075 NM_001945 NP_001936.1


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Images

Immunohistochemistry - Paraffin

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human PANC1 cell lysate. Anti-HBEGF antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

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