Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human HAPLN4. Using a colorimetric detection system, the detection limit for rhHAPLN4 is approximately 1 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 5 ng/well.
Reconstitute in deionized water
For research use only. Intended for use by laboratory professionals.
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