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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C804381-100 100 µl (1 mg/ml) $325 
LS-C804381-200 200 µl (1 mg/ml) $375 
HAPLN1 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HAPLN1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HAPLN1 Antibody - Western blot analysis of HAPLN1 using HepG2 whole cells lysates
HAPLN1 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HAPLN1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
HAPLN1 Antibody - Western blot analysis of HAPLN1 using HepG2 whole cells lysates
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Polyclonal HAPLN1 Antibody (IHC, IF, WB) LS‑C804381

Polyclonal HAPLN1 Antibody (IHC, IF, WB) LS‑C804381

Antibody:
Rabbit Polyclonal to Human HAPLN1
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C804381-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HAPLN1
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HAPLN1 antibody LS-C804381 is an unconjugated rabbit polyclonal antibody to HAPLN1 from human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Human HAPLN1
Synonyms
HAPLN1 | Cartilage-linking protein 1 | CRTL1 | Cartilage link protein | Cartilage linking protein 1 | Cartilage-link protein | Proteoglycan link protein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Bovine, Dog, Rabbit, Sheep (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000 - 1:3000)
  • ELISA (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthesized peptide derived from human HAPLN1.
Specificity
HAPLN1 Antibody detects endogenous levels of total HAPLN1.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20 °C for up to 12 months
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAPLN1
P10915 NM_001884 NP_001875.1

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Requested From: United States
Date Requested: 2/25/2020