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Antibodies > H2AFZ / H2A.z > LS-C804385
 
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Catalog Number Size Price
LS-C804385-100 100 µl (1 mg/ml) $379 
LS-C804385-200 200 µl (1 mg/ml) $421 
H2AFZ / H2A.z Antibody - 1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
H2AFZ / H2A.z Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
H2AFZ / H2A.z Antibody - Western blot analysis of Histone H2A.Z using HeLa whole cells lysates
H2AFZ / H2A.z Antibody - 1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
H2AFZ / H2A.z Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
H2AFZ / H2A.z Antibody - Western blot analysis of Histone H2A.Z using HeLa whole cells lysates
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Polyclonal Rabbit anti‑Human H2AFZ / H2A.z Antibody (IHC, IF, WB) LS‑C804385

Polyclonal Rabbit anti‑Human H2AFZ / H2A.z Antibody (IHC, IF, WB) LS‑C804385

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Antibody:
H2AFZ / H2A.z Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804385-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
H2AFZ / H2A.z Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
H2A.z antibody LS-C804385 is an unconjugated rabbit polyclonal antibody to H2A.z (H2AFZ) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human H2AFZ / H2A.z
Synonyms
H2AFZ | H2AZ histone | H2A histone family, member Z | H2A.z | H2AZ | Histone H2A.Z
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Histone H2A.Z.
Specificity
Histone H2A.Z Antibody detects endogenous levels of total Histone H2A.Z.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFZ / H2A.z
P0C0S5 NM_002106 NP_002097.1

Publications (0)

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Featured Products

H2AFZ / H2A.z Antibody - IHC of paraffin-embedded SCC4 xenograft using Histone H2A.Z antibody at 1:100 dilution.
H2AFZ / H2A.z Antibody LS‑C185971
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
H2AFZ / H2A.z Antibody - Immunohistochemical analysis of Histone H2A.Z (AcK5) staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
H2AFZ / H2A.z Antibody (N‑Terminus) LS‑C359008
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Sheep, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
H2AFZ / H2A.z Antibody (Internal) LS‑C153462
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
H2AFZ / H2A.z Antibody - Immunocytochemistry analysis of Acetyl-H2AFZ (K4) Antibody diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
H2AFZ / H2A.z Antibody LS‑C672748
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
H2AFZ / H2A.z Antibody - H2AFZ / Histone H2A. Z antibody Western blot of 721_B Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
H2AFZ / H2A.z Antibody (N‑Terminus) LS‑C145623
Species: Human
Applications: Western blot
VIL1 / Villin Antibody - Anti-VIL1 / Villin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ VIL1 / Villin Antibody LS‑B2581
Species: Human
Applications: IHC, IHC - Paraffin
See all H2AFZ / H2A.z Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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