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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GTF2I / TFII I Antibody (aa208‑257, Biotin) LS‑C426996
TFII I antibody LS-C426996 is a biotin-conjugated rabbit polyclonal antibody to TFII I (GTF2I) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C426996-100
100 µl (1 mg/ml)
$385

Popular GTF2I / TFII I Products

Anti-GTF2I / TFII I antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Western blot analysis of extracts of 293 cells, using GTF2I antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
Detection of Human and Mouse GTF2I/TFII-I by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GTF2I/TFII-I used at a dilution of 1:200 (1 Detection: DAB.
Species: Human, Mouse, Dog, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

GTF2I / TFII I Antibody (aa208-257, Biotin) for IHC, WB/Western LS-C426996

Specifications

Target
Human GTF2I / TFII I
Synonyms
GTF2I, BAP-135, BAP135, BTK-associated protein, 135kD, BTK-associated protein 135, DIWS, IB291, SRF-Phox1-interacting protein, WBSCR6, TFII I, TFII-I, BTKAP1, WBS, GTFII-I, SPIN
Host
Rabbit
Reactivity
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Drosophila (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GTF2I / TFII I antibody was raised against synthetic peptide located between aa208-257 of human GTF2I (Q75M86, NP_127493). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Turkey, Drosophila (100%); Opossum, Chicken, Platypus (92%).
Epitope
aa208-257
Specificity
Human GTF2I / TFII-I
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About GTF2I / TFII I
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). P78347 NM_032999 NP_127492.1


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Images

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GTF2I / TFII-I antibody LS-C29903 was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/16/2018

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