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Antibodies > GRIK1 / GLUR5 > LS-C344060
 
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Catalog Number Size Price
LS-C344060-10 10 µg $318 
LS-C344060-100 100 µg (0.5 mg/ml) $470 
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of mouse brain tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - Western blot analysis of GRIK1 using anti-GRIK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 104KD. The expected band size for GRIK1 is at 104KD.
GRIK1 / GLUR5 Antibody - GRIK1 antibody Western blot. All lanes: Anti GRIK1 at 0.5 ug/ml. Lane 1: Mouse Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: SHG Whole Cell Lysate at 40 ug. Predicted band size: 104 kD. Observed band size: 104 kD.
GRIK1 / GLUR5 Antibody - GRIK1 antibody Western blot. All lanes: Anti GRIK1 at 0.5 ug/ml. WB: Recombinant Human GRIK1 Protein 0.5ng. Predicted band size: 40 kD. Observed band size: 40 kD.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of mouse brain tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - IHC analysis of GRIK1 using anti-GRIK1 antibody. GRIK1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GRIK1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GRIK1 / GLUR5 Antibody - Western blot analysis of GRIK1 using anti-GRIK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 104KD. The expected band size for GRIK1 is at 104KD.
GRIK1 / GLUR5 Antibody - GRIK1 antibody Western blot. All lanes: Anti GRIK1 at 0.5 ug/ml. Lane 1: Mouse Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: SHG Whole Cell Lysate at 40 ug. Predicted band size: 104 kD. Observed band size: 104 kD.
GRIK1 / GLUR5 Antibody - GRIK1 antibody Western blot. All lanes: Anti GRIK1 at 0.5 ug/ml. WB: Recombinant Human GRIK1 Protein 0.5ng. Predicted band size: 40 kD. Observed band size: 40 kD.
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Polyclonal Rabbit anti‑Human GRIK1 / GLUR5 Antibody (aa271‑450, WB) LS‑C344060

Polyclonal Rabbit anti‑Human GRIK1 / GLUR5 Antibody (aa271‑450, WB) LS‑C344060

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Note: This antibody replaces LS-C388514
Antibody:
GRIK1 / GLUR5 Rabbit anti-Human Polyclonal (aa271-450) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344060-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GRIK1 / GLUR5 Rabbit anti-Human Polyclonal (aa271-450) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GLUR5 antibody LS-C344060 is an unconjugated rabbit polyclonal antibody to GLUR5 (GRIK1) (aa271-450) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human GRIK1 / GLUR5
Synonyms
GRIK1 | GluR-5 | GLUR5 | EEA3 | GluK1 | Glutamate receptor 5 | EAA3 | GLR5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human GRIK1 recombinant protein (Position: R271-I450). Human GRIK1 shares 88% and 94% amino acid (aa) sequences identity with mouse and rat GRIK1, respectively.
Epitope
aa271-450
Specificity
Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GRIK1 / GLUR5
P39086 NM_000830 NP_000821.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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