Target
Mouse Gr-1 Antigen
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
R. Phycoerythrin.
Also available
Unconjugated.
Purification
Protein G purified
Immunogen
Normal murine bone marrow cells
Specificity
Recognizes the mouse Gr-1 antigen, a 21-25kD cell surface protein that belongs to the Ly-6 family of proteins. Clone Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated. The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported. Clone RB6-8C5 has been reported to deplete mouse mature neutrophils in vivo. XXX recommend the use of MCA2387EL for functional studies.
Applications
- Flow Cytometry (1:1 - 1:5)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee