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Catalog Number Size Price
LS-C344054-10 10 µg $318 
LS-C344054-100 100 µg (0.5 mg/ml) $470 
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody IHC-paraffin: Rat Pancreas Tissue.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody IHC-paraffin: Mouse Pancreas Tissue.
GPX1 / Glutathione Peroxidase Antibody - Western blot analysis of GPX1 using anti-GPX1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates. Lane 3: human A549 whole cell lysates. Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22KD. The expected band size for GPX1 is at 22KD.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody Western blot. All lanes: Anti GPX1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: Rat Spleen Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Predicted band size: 22,44 kD. Observed band size: 22,44 kD.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody Western blot. All lanes: Anti GPX1 at 0.5 ug/ml. WB: Recombinant Human GPX1 Protein 0.5ng. Predicted band size: 36 kD. Observed band size: 36 kD.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody IHC-paraffin: Rat Pancreas Tissue.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody IHC-paraffin: Mouse Pancreas Tissue.
GPX1 / Glutathione Peroxidase Antibody - Western blot analysis of GPX1 using anti-GPX1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates. Lane 3: human A549 whole cell lysates. Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22KD. The expected band size for GPX1 is at 22KD.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody Western blot. All lanes: Anti GPX1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: Rat Spleen Tissue Lysate at 50 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Predicted band size: 22,44 kD. Observed band size: 22,44 kD.
GPX1 / Glutathione Peroxidase Antibody - GPX1 antibody Western blot. All lanes: Anti GPX1 at 0.5 ug/ml. WB: Recombinant Human GPX1 Protein 0.5ng. Predicted band size: 36 kD. Observed band size: 36 kD.
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Polyclonal Rabbit anti‑Human GPX1 / Glutathione Peroxidase Antibody (aa116‑146, IHC, WB) LS‑C344054

Polyclonal Rabbit anti‑Human GPX1 / Glutathione Peroxidase Antibody (aa116‑146, IHC, WB) LS‑C344054

Note: This antibody replaces LS-C388524
Antibody:
GPX1 / Glutathione Peroxidase Rabbit anti-Human Polyclonal (aa116-146) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Orangutan, Gibbon, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344054-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GPX1 / Glutathione Peroxidase Rabbit anti-Human Polyclonal (aa116-146) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Orangutan, Gibbon, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Glutathione Peroxidase antibody LS-C344054 is an unconjugated rabbit polyclonal antibody to Glutathione Peroxidase (GPX1) (aa116-146) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human GPX1 / Glutathione Peroxidase
Synonyms
GPX1 | Glutathione peroxidase | GSHPx-1 | Glutathione peroxidase 1 | GPx-1 | GSHPX1 | GPXD
Host
Rabbit
Reactivity
Human, Orangutan, Gibbon, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GPX1(116-146 aa EVNGAGAHPLFAFLREALPAPSDDATALMTD), different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.
Epitope
aa116-146
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPX1 / Glutathione Peroxidase
P07203 NM_000581 NP_000572.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024