GPRC5C antibody LS-A4082 is an unconjugated rabbit polyclonal antibody to human GPRC5C. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Also available conjugated with AP
GPRC5C antibody was raised against synthetic 18 amino acid peptide from C-terminal cytoplasmic domain of human GPRC5C. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Orangutan, Gibbon, Monkey, Marmoset (94%); Mouse, Rat, Dog, Bovine, Hamster, Elephant, Horse, Rabbit, Opossum, Xenopus (89%); Bat, Turkey, Lizard (83%).
Human GPRC5C. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A4082 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A4082 was determined to be 3-32 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.