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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR75 Antibody (C‑Terminus) IHC‑plus™ LS‑A1593
GPR75 antibody LS-A1593 is an unconjugated rabbit polyclonal antibody to GPR75 from human, bat, bovine and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A1593-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular GPR75 Products

Anti-GPR75 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1590 concentration 3-7 ug/ml.
Species: Human, Monkey, Bat, Horse, Xenopus
Applications: IHC, IHC - Paraffin
Anti-GPR75 antibody LS-A1594 IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Rat, Bat, Bovine, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin
Antibody
Species: Human, Mouse, Rat
Applications: ICC, Western blot
Immunofluorescent analysis of GPR75 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human testis using antibody at dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

GPR75 Antibody (C-Terminus) for IHC IHC-plus™ LS-A1593

Specifications

Target
Human GPR75
Synonyms
GPR75, G protein-coupled receptor 75, GPR-chr2, WI31133, GPRchr2, WI-31133
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 - 25 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR75 antibody was raised against synthetic 19 amino acid peptide from C-terminus of human GPR75. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Bovine, Bat, Elephant, Horse, Rabbit, Pig (100%); Mouse, Rat, Hamster, Panda, Turkey (95%); Opossum, Chicken, Lizard, Xenopus (89%); Zebrafish (84%).
Blocking Peptide
LS-E28345 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1593. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human GPR75. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GPR75
G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor. O95800 NM_006794 NP_006785.1


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Images

Immunohistochemistry

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Immunohistochemistry

Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.
Anti-GPR75 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A1593 dilution 10-25 ug/ml.

Requested From: United States
Date Requested: 10/15/2018

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