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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR4 Antibody (C‑Terminus) IHC‑plus™ LS‑A145
GPR4 antibody LS-A145 is an unconjugated rabbit polyclonal antibody to GPR4 from human, dog and monkey. Validated for ICC and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A145-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular GPR4 Products

Anti-GPR4 antibody LS-A143 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin
Anti-GPR4 antibody LS-A144 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Dog, Pig
Applications: IHC, IHC - Paraffin
Antibody
Species: Human
Applications: IHC, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Rat
Applications: Western blot

Product Description

GPR4 antibody LS-A145 is an unconjugated rabbit polyclonal antibody to GPR4 from human, dog and monkey. Validated for ICC and IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human GPR4
Synonyms
GPR4, G-protein coupled receptor 19, G-protein coupled receptor 4, Gpr6c, G protein-coupled receptor 4
Host
Rabbit
Reactivity
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (24 µg/ml)
  • ICC
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR4 antibody was raised against synthetic 19 amino acid peptide from C-terminus of human GPR4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Panda (100%); Mouse, Rat, Bovine, Bat, Pig (95%); Rabbit (89%).
Blocking Peptide
LS-E28286 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A145. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human GPR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GPR4
GPR4 is a Lysophospholipid/Lysosphingolipid receptor. Recently, GPR4 has been shown to act as a proton-sensing receptor stimulating inositol phosphate formation (Ludwig et al. 2003). P46093 NM_005282 NP_005273.1


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Images

Immunohistochemistry

Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR4 antibody LS-A145 IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry

Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-GPR4 antibody LS-A145 immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Requested From: United States
Date Requested: 10/16/2018

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