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Catalog Number Size Price
LS-C744975-25 25 µl (2.1 mg/ml) $275 
GPC1 / Glypican Antibody - Immunohistochemistry of rabbit anti-Glypican antibody. Tissue: skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Glypican antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.
GPC1 / Glypican Antibody - Western Blot of rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.
GPC1 / Glypican Antibody - Immunohistochemistry of rabbit anti-Glypican antibody. Tissue: skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Glypican antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.
GPC1 / Glypican Antibody - Western Blot of rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.
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Polyclonal Rabbit anti‑Human GPC1 / Glypican Antibody (Internal, IHC, WB) LS‑C744975

Polyclonal Rabbit anti‑Human GPC1 / Glypican Antibody (Internal, IHC, WB) LS‑C744975

Antibody:
GPC1 / Glypican Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744975-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GPC1 / Glypican Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Glypican antibody LS-C744975 is an unconjugated rabbit polyclonal antibody to Glypican (GPC1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human GPC1 / Glypican
Synonyms
GPC1 | Glypican 1 | Glypican | Glypican-1 | Glypican proteoglycan 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-Glypican-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
Epitope
Internal
Applications
  • IHC (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPC1 / Glypican
P35052 NM_002081 NP_002072.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/13/2021