Target
Aspergillus niger gox / Glucose Oxidase
Reactivity
Aspergillus niger
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Glucose Oxidase [Aspergillus niger].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:3000)
- ELISA (1:5500 - 1:25000)
- Dot Blot
Usage
GLUCOSE OXIDASE antibody has been assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2000 to 1:10000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About gox / Glucose Oxidase