Target
Rabbit IgG + IgA + IgM
Reactivity
Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
TRITC,
Biotin,
FITC,
HRP.
Purification
Purified IgG
Immunogen
Highly purified IgG and pools of homogenous IgA and IgM isolated from rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rabbit immunoglobulins IgG, IgA, IgM, heavy and light chains.
Applications
- Immunofluorescence
- ELISA
- Dot Blot
Usage
The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rabbit plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates. in radioimmunoassay. for the production of immunoconjugates with a selected marker. to prepare immunoaffinity adsorbents by coupling to an artificial carrier. in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent. in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee