Rabbit IgG Fc

Goat

Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

FITC. Also available Unconjugated or conjugated with HRP, Biotin.

Purified IgG

Unmodified

Pools of purified IgG isolated from rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgG, Fc specific.

• IHC
• IHC - Frozen
• ICC
• Immunofluorescence
• ELISA
• Immunodiffusion
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is restricted to the Fc part of the IgG molecule which expresses strict (class) specificity. In immunoelectrophoresis and radial immunodiffusion using various antiserum concentrations against rabbit serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgG used as immunogens. It does not react with IgA, IgG/Fab fragments and IgM or any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.