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Goat Anti‑HRP / Horseradish Peroxidase Secondary Antibody LS‑C153963

Catalog Number Size Price
LS-C153963-2 2 ml (45 mg/ml) $425 
Anti-Peroxidase Polyclonal Antibody-Western blot. Goat anti-Peroxidase antibody was used to detect horse radish peroxidase under reducing and non-reducing conditions. Reduced samples of purified horse radish peroxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti-Peroxidase antibody (1:5K in MB-0070, overnight at 4C). Primary antibody was detected with Dylight 649 conjugated Donkey anti-Goat (1:10K 1.5 hr RT in MB-070 and imaged on the Bio-Rad VersaDoc imaging system.

Goat Anti‑HRP / Horseradish Peroxidase Secondary Antibody LS‑C153963

Antibody:
Goat Polyclonal to Horseradish HRP / Horseradish Peroxidase
Application:
WB, ELISA
Reactivity:
Horseradish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153963-2
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Horseradish HRP / Horseradish Peroxidase
Application:
WB, ELISA
Reactivity:
Horseradish
Format:
Unconjugated, Unmodified

Specifications

Description
Horseradish Peroxidase antibody LS-C153963 is an unconjugated goat polyclonal antibody. Validated for ELISA and WB.
Target
HRP / Horseradish Peroxidase
Host
Goat
Reactivity
Horseradish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • Western blot (1:900 - 1:4000)
  • ELISA
Immunogen
Peroxidase [Horseradish].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Usage
This product has been assayed against 1.0 ug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9000 to 1:40000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9000 to 1:40000 of the reconstitution concentration is suggested for this product.
Presentation
0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
2 ml deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Product
Species: Horseradish
Applications: IHC, Western blot, ELISA
Product
Species: Horseradish
Applications: Immunodiffusion
Western blot of  purified horse radish peroxidase sample; non-reduced sample, immunostained by mAb HP-03 and anti-goat anti-mouse IgG (H+L)-HRP conjugate.
Species: Horseradish
Applications: ICC, Western blot, ELISA
Product
Species: Horseradish
Applications: IHC, IHC - Paraffin, ELISA

Requested From: United States
Date Requested: 8/18/2019
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