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Goat Anti‑GST / Glutathione S‑Transferase Secondary Antibody (AP) LS‑C154195

Catalog Number Size Price
LS-C154195-1 1 mg (1 mg/ml) $605 
Anti-GST Polyclonal Antibody-Western blot. Goat anti-GST antibody ( was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For blot on the left, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti-GST (1:5K in MB-0070, ON 4 C). Primary antibody was detected with Dylight 649 conjugated Donkey anti-Goat (1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc imaging system. Blot on right shows a repeat western blot with the same samples (~1 ug per lane, reduced (R) and non-reduced (NR) probed 1:1000 dilution of primary antibody and detection using Dylight 549 conjugated Donkey anti-goat (1:10K 1.5 hr RT in MB-070). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Goat Anti‑GST / Glutathione S‑Transferase Secondary Antibody (AP) LS‑C154195

Antibody:
Goat Polyclonal to Schistosoma japonicum GST / Glutathione S-Transferase
Application:
IHC, WB, ELISA
Reactivity:
Schistosoma japonicum
Format:
Alkaline Phosphatase, Unmodified
Price
Catalog Number
$605 
LS-C154195-1
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Schistosoma japonicum GST / Glutathione S-Transferase
Application:
IHC, WB, ELISA
Reactivity:
Schistosoma japonicum
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
Glutathione S-Transferase antibody LS-C154195 is an AP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Schistosoma japonicum GST / Glutathione S-Transferase
Host
Goat
Reactivity
Schistosoma japonicum (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Glutathione-S-Transferase [Schistosoma japonicum].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Usage
Anti-GST Antibody is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.1% sodium azide, sterile filtered
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Western blot of Mouse-anti-GST antibody. Lane 1: molecular weight. Lane 2: Mouse-anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha-1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10000 for 30 min at RT. Block: MB-070 overnight at 4C.
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Requested From: United States
Date Requested: 8/17/2019
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