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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GM2A Antibody (N‑Terminus) LS‑C111076
GM2A antibody LS-C111076 is an unconjugated rabbit polyclonal antibody to human GM2A. Validated for WB.
Catalog
Size
Price
LS-C111076-50
50 µg
$365

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Product Description

GM2A antibody LS-C111076 is an unconjugated rabbit polyclonal antibody to human GM2A. Validated for WB.

Specifications

Target
Human GM2A
Synonyms
GM2A, Ganglioside GM2 activator, GM2 ganglioside activator, GM2-AP, SAP-3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
GM2A antibody was raised against synthetic peptide from N-Terminus of human GM2A (P17900, NP_000396). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey (85%).
Blocking Peptide
LS-E16757 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111076. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human GM2A
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GM2A
The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. P17900 NM_000405 NP_000396.2


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Images

Western blot

GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GM2A antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/14/2018

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