Target
Human GM2A
Synonyms
GM2A | Ganglioside GM2 activator | GM2 ganglioside activator | GM2-AP | SAP-3
Reactivity
Human, Monkey, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence KRLGCIKIAAS, from the internal region of the protein sequence according to NP_000396.2.
Specificity
Human GM2A. This antibody is expected to recognize isoform 1 (NP_000396.2) only.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: In transfected HEK293 transiently expressing full-length Human GM2A (myc and DYKDDDDK tagged), a band of approx. 28kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee