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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745295-25 | 25 µl (1 mg/ml) | $304 |
![GLI2 Antibody - Western blot using the affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/gli2-antibody-ls-c745295/445708_3396240.jpg)
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Polyclonal Rabbit anti‑Human GLI2 Antibody (IHC, WB) LS‑C745295
Polyclonal Rabbit anti‑Human GLI2 Antibody (IHC, WB) LS‑C745295
Antibody:
GLI2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
GLI2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Specifications
Description
GLI2 antibody LS-C745295 is an unconjugated rabbit polyclonal antibody to GLI2 from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB.
Target
Human GLI2
Synonyms
GLI2 | GLI-Kruppel family member GLI2 | HPE9 | Gli-2 | Oncogene GLI2 | Tax helper protein 2 | THP | Zinc finger protein GLI2 | Tax helper protein | THP2 | GLI family zinc finger 2 | Tax helper protein 1 | THP1
Host
Rabbit
Reactivity
Human, Chimpanzee
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).
Specificity
A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
Applications
- IHC (1:500 - 1:2000)
- Western blot (1:500 - 1:2000)
- ELISA (1:2000 - 1:12000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GLI2
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Featured Products
Species:
Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Chimpanzee
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
