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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C14550-100 100 µg $635 
Antibody

Polyclonal Rabbit anti‑Mouse GJA5 / CX40 / Connexin 40 Antibody (C‑Terminus, IHC, IF, WB) LS‑C14550

Polyclonal Rabbit anti‑Mouse GJA5 / CX40 / Connexin 40 Antibody (C‑Terminus, IHC, IF, WB) LS‑C14550

Antibody:
Rabbit Polyclonal to Mouse GJA5 / CX40 / Connexin 40
Application:
IHC, IHC-Fr, IF, WB, IP
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C14550-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Mouse GJA5 / CX40 / Connexin 40
Application:
IHC, IHC-Fr, IF, WB, IP
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Connexin 40 antibody LS-C14550 is an unconjugated rabbit polyclonal antibody to mouse Connexin 40 (GJA5 / CX40) (C-Terminus). Validated for IF, IHC, IP and WB.
Target
Mouse GJA5 / CX40 / Connexin 40
Synonyms
GJA5 | Connexin 40 | Connexin-40 | Gap junction alpha-5 protein | ATFB11 | CX40 | Gap junction protein alpha 5
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunofluorescence (1 - 3 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from the C-Terminus of the mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid.
Epitope
C-Terminus
Specificity
Reacts with the mouse Connexin 40 protein. On Western Blots, it identifies the target band at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence. Species cross-reactivity: Cross-reactivity with rat and human CX40 proteins is expected based on amino acid sequence homology.
Usage
Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunofluorescence: 1-3 ug/ml. Immunoprecipitation: 7 ug/reaction. Immunohistochemistry (Frozen sections): 1-3 ug/ml; For IHC, mice were perfused transcardially with 3 ml pre-fixative (50 mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin),. followed by 40 ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash (10% sucrose in 25 mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25 mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GJA5 / CX40 / Connexin 40
P36382 NM_005266 NP_005257.2

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Requested From: United States
Date Requested: 7/6/2020