Mouse GJA5 / CX40 / Connexin 40
GJA5 | Connexin 40 | Connexin-40 | Gap junction alpha-5 protein | ATFB11 | CX40 | Gap junction protein alpha 5
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Frozen
- Immunofluorescence (1 - 3 µg/ml)
- Western blot (1 - 3 µg/ml)
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Synthetic peptide derived from the C-Terminus of the mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid.
Reacts with the mouse Connexin 40 protein. On Western Blots, it identifies the target band at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence. Species cross-reactivity: Cross-reactivity with rat and human CX40 proteins is expected based on amino acid sequence homology.
Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 ug/ml. Immunofluorescence: 1-3 ug/ml. Immunoprecipitation: 7 ug/reaction. Immunohistochemistry (Frozen sections): 1-3 ug/ml; For IHC, mice were perfused transcardially with 3 ml pre-fixative (50 mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin),. followed by 40 ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10 ml sucrose wash (10% sucrose in 25 mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10% sucrose, 25 mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
PBS, pH 7.4, 0.1% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.
About GJA5 / CX40 / Connexin 40