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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GCN1 / GCN1L1 Antibody (aa2621‑2671) LS‑C288620 Best Seller
GCN1L1 antibody LS-C288620 is an unconjugated rabbit polyclonal antibody to GCN1L1 (GCN1) from human, mouse and rat. Validated for IP and WB.
Catalog
Size
Price
LS-C288620-100
100 µl (0.2 mg/ml)
$395

Popular GCN1 / GCN1L1 Products

Western blot analysis of extracts of various cell lines, using GCN1L1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
Species: Human
Applications: Western blot

Product Description

GCN1L1 antibody LS-C288620 is an unconjugated rabbit polyclonal antibody to GCN1L1 (GCN1) from human, mouse and rat. Validated for IP and WB.
About GCN1 / GCN1L1
Acts as a translation activator that mediates translational control and perform an EF3-related function on the ribosome by regulating GCN2 protein kinase (EIF2AK1-4) activity. Q92616 D86973 BAA13209.2

GCN1L1 Antibody, HsGCN1 Antibody, GCN1L Antibody, PRIC295 Antibody, Translational activator GCN1 Antibody, GCN1 Antibody, GCN1-like protein 1 Antibody, KIAA0219 Antibody


Specifications

Target
Human GCN1 / GCN1L1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
Epitope
aa2621-2671
Specificity
Region between residue 2621 and 2671 of human general control of amino-acid synthesis 1-like 1 using the numbering given in entry NP_006827.1 (GeneID 10985).
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.

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Customer Reviews (0)


Images

Western blot

Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western blot

Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western blot

Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western blot

Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse and Rat GCN1L1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human GCN1L1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Requested From: United States
Date Requested: 9/21/2018

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