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Antibodies > GATA3 > LS-C356055
 
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Catalog Number Size Price
LS-C356055-100 100 µl (1 mg/ml) $299 
GATA3 Antibody - Immunohistochemical analysis of GATA3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA3 Antibody - Immunofluorescent analysis of GATA3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GATA3 Antibody - Western blot analysis of GATA3 expression in SHSY5Y (A) whole cell lysates.
GATA3 Antibody - Immunohistochemical analysis of GATA3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA3 Antibody - Immunofluorescent analysis of GATA3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GATA3 Antibody - Western blot analysis of GATA3 expression in SHSY5Y (A) whole cell lysates.
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Polyclonal Rabbit anti‑Human GATA3 Antibody (Internal, IF, WB) LS‑C356055

Polyclonal Rabbit anti‑Human GATA3 Antibody (Internal, IF, WB) LS‑C356055

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Antibody:
GATA3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Bovine, Pig, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C356055-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GATA3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Bovine, Pig, Chicken, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
GATA3 antibody LS-C356055 is an unconjugated rabbit polyclonal antibody to GATA3 (Internal) from human. It is reactive with human, mouse, bovine and other species. Validated for ICC, IF and WB.
Target
Human GATA3
Synonyms
GATA3 | GATA-binding factor 3 | HDRS | GATA binding protein 3 | GATA-binding protein 3 | HDR
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Pig, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human GATA3.
Epitope
Internal
Specificity
Recognizes endogenous levels of GATA3 protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GATA3
P23771 NM_001002295 NP_001002295.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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