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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GAS1 Antibody (aa95‑127) LS‑C166376 Best Seller
GAS1 antibody LS-C166376 is an unconjugated rabbit polyclonal antibody to GAS1 from human and mouse. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C166376-400
400 µl
$305
Description
GAS1 antibody LS-C166376 is an unconjugated rabbit polyclonal antibody to GAS1 from human and mouse. Validated for Flow, IF, IHC and WB.
Target
Human GAS1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1275 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166376. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa95-127
Specificity
This GAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-127 amino acids from the N-terminal region of human GAS1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About GAS1
P54826 NM_002048 NP_002039.2

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Images

Immunohistochemistry

GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).
GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).

Flow Cytometry

GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).
GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).

Flow Cytometry

GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).
GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).

Flow Cytometry

GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GAS1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded mouse heart tissue tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of GAS1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).
GAS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The GAS1 antibody detected the GAS1 protein (arrow).

Flow Cytometry

GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GAS1 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/17/2018
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