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Antibodies > GALK1 / GK1 > LS-C818015
 
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Catalog Number Size Price
LS-C818015-100 100 µl $412 
GALK1 / GK1 Antibody - Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- HG3C-GALK1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
GALK1 / GK1 Antibody - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: GALK1 konckout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
GALK1 / GK1 Antibody - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa. (We are unsure as to the identity of these extra bands.)
GALK1 / GK1 Antibody - GALK1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-GALK1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-GALK1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
GALK1 / GK1 Antibody - Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- HG3C-GALK1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
GALK1 / GK1 Antibody - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: GALK1 konckout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
GALK1 / GK1 Antibody - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa. (We are unsure as to the identity of these extra bands.)
GALK1 / GK1 Antibody - GALK1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-GALK1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-GALK1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
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Polyclonal Rabbit anti‑Human GALK1 / GK1 Antibody (Preservative Free, IF, WB) LS‑C818015

Polyclonal Rabbit anti‑Human GALK1 / GK1 Antibody (Preservative Free, IF, WB) LS‑C818015

Submit Review LSBio Guarantee
Antibody:
GALK1 / GK1 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C818015-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GALK1 / GK1 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
GK1 antibody LS-C818015 is an unconjugated rabbit polyclonal antibody to human GK1 (GALK1). Validated for ICC, IF, IP and WB.
Target
Human GALK1 / GK1
Synonyms
GALK1 | Galactokinase 1 | GALK | GK1 | Galactokinase | Galactose kinase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
Recombinant Human GALK1 Protein
Specificity
Human GALK1
Applications
  • ICC (1:300 - 1:10000)
  • Immunofluorescence (1:300 - 1:10000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GALK1 / GK1
P51570 NM_000154 NP_000145.1

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GALK1 / GK1 Antibody - Immunofluorescent analysis of Galactose Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Species: Human, Mouse, Rat, Dog
Applications: ICC, Immunofluorescence, Western blot
GALK1 / GK1 Antibody - Immunofluorescent analysis of HepG2 cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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GALK1 / GK1 ELISA Kit
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Reactivity: Human, Mouse, Rat
Range: Positive/Negative
See all GALK1 / GK1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/1/2024
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