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Antibodies > GABRA5 > LS-C773945
 
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Catalog Number Size Price
LS-C773945-100 100 µg (1 mg/ml) $478 
GABRA5 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Alpha5 Antibody. (D) Composite.
GABRA5 Antibody - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.
GABRA5 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Alpha5 Antibody. (D) Composite.
GABRA5 Antibody - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.
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Monoclonal Mouse anti‑Human GABRA5 Antibody (clone S415‑24, PerCP, IHC, IF, WB) LS‑C773945

Monoclonal Mouse anti‑Human GABRA5 Antibody (clone S415‑24, PerCP, IHC, IF, WB) LS‑C773945

Submit Review LSBio Guarantee
Antibody:
GABRA5 Mouse anti-Human Monoclonal (PerCP) (S415-24) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:
Price
Catalog Number
$478
LS-C773945-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GABRA5 Mouse anti-Human Monoclonal (PerCP) (S415-24) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:

Specifications

Description
GABRA5 antibody LS-C773945 is a PerCP-conjugated mouse monoclonal antibody to GABRA5 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human GABRA5
Synonyms
GABRA5 | Alpha 5 | GABA A receptor alpha 5
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
S415-24
Conjugations
Peridinin-chlorophyll-protein. Also available conjugated with AP, APC, Atto 390, Atto 594, Biotin, FITC, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-Terminus) of human GABA A Receptor Alpha5.
Specificity
Detects ~55 kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GABRA5
P31644 NM_000810 NP_000801.1

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GABRA5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GABRA5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GABRA5.
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/23/2023
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