GABRA5 antibody LS-C101528 is an unconjugated rabbit polyclonal antibody to GABRA5 from human, mouse, rat and other species. Validated for WB.
Human, Mouse, Rat, Guinea pig, Hamster, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
GABRA5 antibody was raised against synthetic peptide from C-Terminus of human GABRA5 (P31644, NP_000801). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard (100%); Monkey, Elephant, Panda, Dog, Bovine, Horse, Pig, Platypus, Xenopus (92%); Zebrafish (91%); Zebra finch (85%); Gorilla, Bat (81%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101528. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.