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Catalog Number Size Price
LS-C773960-100 100 µg (1 mg/ml) $474 
GABRA2 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody, Clone S399-19. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Alpha2 Antibody. (D) Composite.
GABRA2 Antibody - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.
GABRA2 Antibody - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.
GABRA2 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody, Clone S399-19. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Alpha2 Antibody. (D) Composite.
GABRA2 Antibody - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.
GABRA2 Antibody - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.
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Monoclonal Mouse anti‑Human GABRA2 Antibody (clone S399‑19, AP, IHC, IF, WB) LS‑C773960

Monoclonal Mouse anti‑Human GABRA2 Antibody (clone S399‑19, AP, IHC, IF, WB) LS‑C773960

Antibody:
GABRA2 Mouse anti-Human Monoclonal (AP) (S399-19) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Alkaline Phosphatase, Unmodified
Other formats:
Price
Catalog Number
$474
LS-C773960-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GABRA2 Mouse anti-Human Monoclonal (AP) (S399-19) Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Alkaline Phosphatase, Unmodified
Other formats:

Specifications

Description
GABRA2 antibody LS-C773960 is an AP-conjugated mouse monoclonal antibody to GABRA2 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human GABRA2
Synonyms
GABRA2 | GABA A receptor alpha 2 | GABA(A) receptor, alpha 2
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
S399-19
Conjugations
Alkaline Phosphatase. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein amino acids 350-385 (Cytoplasmic C-Terminus) of rat GABA-A Receptor Alpha2
Specificity
Detects ~55 kDa. Does not cross-react with GABA-A Receptor Alpha1.
Applications
  • IHC
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GABRA2
P47869 NM_000807 NP_000798.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024