Frizzled 1 antibody LS-C6895 is an unconjugated rat monoclonal antibody to mouse Frizzled 1 (FZD1). Validated for ELISA and WB.
Mouse FZD1 / Frizzled 1
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1 - 2 µg/ml)
NS0-derived rmFrizzled-1 extracellular domain.
Recognizes mouse Frizzled-1. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 or -8.
Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. Using a colorimetric detection system, the detection limit for rmFrizzled-1 is ~5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: Used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/well.
PBS, pH 7.2, 5% Trehalose
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.