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Catalog Number Size Price
LS-C313049-10 10 µg $318 
LS-C313049-100 100 µg (0.5 mg/ml) $470 
FSTL3 / FLRG Antibody - Western blot analysis of FSTL3 using anti-FSTL3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.
FSTL3 / FLRG Antibody - WB of FSTL3 / FLRG antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human FSTL3, 39.2KD. (162aa tag+ L52-P251). Lane 1: Recombinant Human FSTL3 Protein 10ng. Lane 2: Recombinant Human FSTL3 Protein 5ng.
FSTL3 / FLRG Antibody - Western blot analysis of FSTL3 using anti-FSTL3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.
FSTL3 / FLRG Antibody - WB of FSTL3 / FLRG antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human FSTL3, 39.2KD. (162aa tag+ L52-P251). Lane 1: Recombinant Human FSTL3 Protein 10ng. Lane 2: Recombinant Human FSTL3 Protein 5ng.
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Polyclonal Rabbit anti‑Human FSTL3 / FLRG Antibody (aa145‑159, WB) LS‑C313049

Polyclonal Rabbit anti‑Human FSTL3 / FLRG Antibody (aa145‑159, WB) LS‑C313049

Note: This antibody replaces LS-C388468
Antibody:
FSTL3 / FLRG Rabbit anti-Human Polyclonal (aa145-159) Antibody
Application:
WB
Reactivity:
Human, Monkey, Bat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313049-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FSTL3 / FLRG Rabbit anti-Human Polyclonal (aa145-159) Antibody
Application:
WB
Reactivity:
Human, Monkey, Bat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
FLRG antibody LS-C313049 is an unconjugated rabbit polyclonal antibody to FLRG (FSTL3) (aa145-159) from human. It is reactive with human, bat, bovine and other species. Validated for WB.
Target
Human FSTL3 / FLRG
Synonyms
FSTL3 | FLRG | Follistatin-related protein 3 | Follistatin-like protein 3 | FSRP
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FSTL3(145-159 aa ECELRAARCRGHPDL), different from the related rat and mouse sequences by one amino acid.
Epitope
aa145-159
Specificity
Expressed in a wide range of tissues. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FSTL3 / FLRG
O95633 NM_005860 NP_005851.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/30/2024