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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXI1 Antibody (clone 2C1) LS‑C175141
FOXI1 antibody LS-C175141 is an unconjugated mouse monoclonal antibody to human FOXI1. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C175141-100
100 µl (1 mg/ml)
$335
Description
FOXI1 antibody LS-C175141 is an unconjugated mouse monoclonal antibody to human FOXI1. Validated for Flow, IF, IHC and WB.
Target
Human FOXI1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2C1]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXI1 antibody was raised against human recombinant protein fragment corresponding to amino acids 111-350 of human FOXI1 (NP_036320) produced in E. coli.
Specificity
Human FOXI1
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FOXI1
Q12951 NM_012188 NP_036320.2

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Images

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.

Immunofluorescence

Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.
Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.

Immunofluorescence

Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.
Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.

Immunofluorescence

Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.
Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.

Immunofluorescence

Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.
Anti-FOXI1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 11/21/2018
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