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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXA3 Antibody (aa201-250) IHC-plus™ LS-B947

Note: This antibody replaces LS-C29753
Anti-FOXA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXA3 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FOXA3 antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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FOXA3 Antibody (aa201-250) IHC-plus™ LS-B947

Note: This antibody replaces LS-C29753
Antibody:
Rabbit Polyclonal to Human FOXA3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Bovine, Dog, Guinea pig, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B947-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FOXA3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Bovine, Dog, Guinea pig, Pig, Rabbit, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FOXA3 antibody LS-B947 is an unconjugated rabbit polyclonal antibody to FOXA3 from human, bovine, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human FOXA3
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Dog, Guinea pig, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXA3 antibody was raised against synthetic peptide located between aa201-250 of human FOXA3 (P55318, NP_004488). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Pig, Opossum, Guinea pig (100%); Mouse, Rat, Horse (92%); Rabbit (84%).
Blocking Peptide
LS-E11150 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B947. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa201-250
Specificity
Human FOXA3
Usage
Immunohistochemistry: LS-B947 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B947 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXA3
P55318 NM_004497 NP_004488.2

Validation

Anti-FOXA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-FOXA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXA3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-FOXA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-FOXA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 5/26/2019
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