Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C407836-10 10 µg $318 
LS-C407836-100 100 µg $470 
FLOT2 / Flotillin 2 Antibody - IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FLOT2 / Flotillin 2 Antibody - IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FLOT2 / Flotillin 2 Antibody - IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody Western blot. All lanes: Anti Flotillin 2 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Lung Tissue Lysate at 50 ug. Lane 3: Mouse Liver Tissue Lysate at 50 ug. Lane 4: Mouse Lung Tissue Lysate at 50 ug. Lane 5: Mouse Testis Tissue Lysate at 50 ug. Lane 6: Human Placenta Tissue Lysate at 50 ug. Lane 7: A375 Whole Cell Lysate at 40 ug. Lane 8: HELA Whole Cell Lysate at 40 ug. Lane 9: 22RV1 Whole Cell Lysate at 40 ug. Lane 10: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 47 kD. Observed band size: 47 kD.
FLOT2 / Flotillin 2 Antibody - IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FLOT2 / Flotillin 2 Antibody - IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FLOT2 / Flotillin 2 Antibody - IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody. Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Flotillin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
FLOT2 / Flotillin 2 Antibody - Flotillin 2 antibody Western blot. All lanes: Anti Flotillin 2 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Lung Tissue Lysate at 50 ug. Lane 3: Mouse Liver Tissue Lysate at 50 ug. Lane 4: Mouse Lung Tissue Lysate at 50 ug. Lane 5: Mouse Testis Tissue Lysate at 50 ug. Lane 6: Human Placenta Tissue Lysate at 50 ug. Lane 7: A375 Whole Cell Lysate at 40 ug. Lane 8: HELA Whole Cell Lysate at 40 ug. Lane 9: 22RV1 Whole Cell Lysate at 40 ug. Lane 10: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 47 kD. Observed band size: 47 kD.
1 of 7
2 of 7
3 of 7
4 of 7
5 of 7
6 of 7
7 of 7

Polyclonal Rabbit anti‑Human FLOT2 / Flotillin 2 Antibody (aa169‑344, IHC, WB) LS‑C407836

Polyclonal Rabbit anti‑Human FLOT2 / Flotillin 2 Antibody (aa169‑344, IHC, WB) LS‑C407836

Antibody:
FLOT2 / Flotillin 2 Rabbit anti-Human Polyclonal (aa169-344) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407836-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FLOT2 / Flotillin 2 Rabbit anti-Human Polyclonal (aa169-344) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Flotillin 2 antibody LS-C407836 is an unconjugated rabbit polyclonal antibody to Flotillin 2 (FLOT2) (aa169-344) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human FLOT2 / Flotillin 2
Synonyms
FLOT2 | ECS-1 | Epidermal surface antigen | ECS1 | ESA | ESA1 | Flotillin 2 | Flotillin-2 | M17S1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.
Epitope
aa169-344
Specificity
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FLOT2 / Flotillin 2
Q14254 NM_004475 NP_004466.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024