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Catalog Number	Size	Price
LS-C662134-10	10 µg	$318
LS-C662134-100	100 µg	$470

FH / Fumarase / MCL Antibody - Immunohistochemistry - Anti-FH/Fumarase Picoband Antibody

FH / Fumarase / MCL Antibody - IF analysis of FH using anti-FH antibody FH was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-FH Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

FH / Fumarase / MCL Antibody - Western blot - Anti-FH/Fumarase Picoband Antibody

FH / Fumarase / MCL Antibody - Flow Cytometry analysis of PC-3 cells using anti-FH antibody. Overlay histogram showing PC-3 cells stained with anti-FH antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FH Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

FH / Fumarase / MCL Antibody - Flow Cytometry analysis of A431 cells using anti-FH antibody. Overlay histogram showing A431 cells stained with anti-FH antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FH Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human FH / Fumarase / MCL Antibody (IHC, WB) LS‑C662134

Name: Anti-FH / Fumarase / MCL Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662134-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

FH / Fumarase / MCL Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662134-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FH / Fumarase / MCL Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

MCL antibody LS-C662134 is an unconjugated rabbit polyclonal antibody to human MCL (FH / Fumarase). Validated for IHC and WB.

Target

Human FH / Fumarase / MCL

Synonyms

See All FH / Fumarase / MCL Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence of human FH (YDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK).

Specificity

Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology.

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FH / Fumarase / MCL

Uniprot: P07954 NCBI: NM_000143 NP_000134.2