Immunohistochemistry: LS-B3447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3447 was determined to be 1:200.
Ascitic fluid containing 0.03% sodium azide
Short term: store at 4°C. Long term: store at -20°C.
This antibody carries the LSBio 100% Guarantee.