FGFR4 antibody LS-B3447 is an unconjugated mouse monoclonal antibody to human FGFR4. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:200)
- IHC - Frozen
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
FGFR4 antibody was raised against purified recombinant fragment of FGFR4 expressed in E. coli.
Immunohistochemistry: LS-B3447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3447 was determined to be 1:200.
Ascitic fluid containing 0.03% sodium azide
Short term: 4°C; Long term: -20°C.
For research use only.