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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FGFR4 Antibody (clone 4H2B10B2) IHC-plus™ LS-B3447

Note: This antibody replaces LS-C41492, LS-C171378
Anti-FGFR4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot using FGFR4 mouse monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FGFR4 cDNA (2).
Anti-FGFR4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot using FGFR4 mouse monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY FGFR4 cDNA (2).
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FGFR4 Antibody (clone 4H2B10B2) IHC-plus™ LS-B3447

Note: This antibody replaces LS-C41492, LS-C171378
Antibody:
Mouse Monoclonal to Human FGFR4
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3447-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human FGFR4
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FGFR4 antibody LS-B3447 is an unconjugated mouse monoclonal antibody to human FGFR4. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human FGFR4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
4H2B10B2
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • IHC - Frozen
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FGFR4 antibody was raised against purified recombinant fragment of FGFR4 expressed in E. coli.
Specificity
Human
Usage
Immunohistochemistry: LS-B3447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3447 was determined to be 1:200.
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FGFR4
P22455 NM_002011 NP_002002.3

Validation

Anti-FGFR4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-FGFR4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-FGFR4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Featured Products

Reactivity: Rat
Range: 6.25-400 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Product
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation

Requested From: United States
Date Requested: 5/26/2019
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