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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FFAR2 / GPR43 Antibody (Internal, Azide-free, HRP) IHC-plus™ LS-A101529

Anti-GPR43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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FFAR2 / GPR43 Antibody (Internal, Azide-free, HRP) IHC-plus™ LS-A101529

Antibody:
Rabbit Polyclonal to Human FFAR2 / GPR43
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Horseradish Peroxidase, Azide-free
Other formats:
Price
Catalog Number
$650 
LS-A101529-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FFAR2 / GPR43
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Horseradish Peroxidase, Azide-free
Other formats:

Specifications

Description
GPR43 antibody LS-A101529 is an HRP-conjugated rabbit polyclonal antibody to human GPR43 (FFAR2). Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Target
Human FFAR2 / GPR43
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with AP, APC, Biotin, FITC, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FFAR2 / GPR43 antibody was raised against synthetic 17 amino acid peptide from internal region of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (94%).
Blocking Peptide
LS-E28340 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A101529. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human GPR43. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A1586 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1586 was determined to be 5 ug/ml.
Presentation
PBS
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FFAR2 / GPR43
O15552 NM_005306 NP_005297.1

Validation

Anti-GPR43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPR43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPR43 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 6/27/2019
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