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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FFAR2 / GPR43 Antibody (C‑Terminus, PE) IHC‑plus™ LS‑A106324
GPR43 antibody LS-A106324 is a PE-conjugated rabbit polyclonal antibody to human GPR43 (FFAR2). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A106324-100
100 µl
$650
Description
GPR43 antibody LS-A106324 is a PE-conjugated rabbit polyclonal antibody to human GPR43 (FFAR2). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human FFAR2 / GPR43
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Phycoerythrin. Also available Unconjugated or conjugated with AP, APC, Biotin, FITC, HRP.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FFAR2 / GPR43 antibody was raised against synthetic 16 amino acid peptide from C-terminus of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (94%).
Epitope
C-Terminus
Specificity
Human GPR43. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except BMI1 (50%).
Usage
Immunohistochemistry: LS-A6598 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A6598 was determined to be 14 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FFAR2 / GPR43
O15552 NM_005306 NP_005297.1

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Immunohistochemistry

Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 12/15/2018
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