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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FE65L1 / APBB2 Antibody (aa475‑524) IHC‑plus™ LS‑B12571
Note: This antibody replaces LS-C200132
APBB2 antibody LS-B12571 is an unconjugated rabbit polyclonal antibody to APBB2 (FE65L1) from human and mouse. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B12571-100
100 µl (1 mg/ml)
$505
Description
APBB2 antibody LS-B12571 is an unconjugated rabbit polyclonal antibody to APBB2 (FE65L1) from human and mouse. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FE65L1 / APBB2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FE65L1 / APBB2 antibody was raised against synthetic peptide from human APBB2. (AA Range: 475-524)
Epitope
aa475-524
Specificity
APBB2 Antibodyantibody detects endogenous levels of APBB2.
Usage
Western Blot: Predicted MW 83 kDa
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
About FE65L1 / APBB2
Q92870 U62325 AAC50805.1

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Immunohistochemical analysis of APBB2 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, Western blot

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Images

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.
Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.
Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.
Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.
Western blot analysis of the lysates from HepG2 cells using APBB2 antibody.

Requested From: United States
Date Requested: 11/20/2018
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