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Catalog Number Size Price
LS-C748993-20 20 µl $275 
LS-C748993-100 100 µl $389 
FCGR3B / CD16B Antibody - Immunofluorescence analysis of U2OS cells using FCGR3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
FCGR3B / CD16B Antibody - Western blot analysis of extracts of various cell lines, using FCGR3B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
FCGR3B / CD16B Antibody - Immunofluorescence analysis of U2OS cells using FCGR3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
FCGR3B / CD16B Antibody - Western blot analysis of extracts of various cell lines, using FCGR3B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
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Polyclonal Rabbit anti‑Human FCGR3B / CD16B Antibody (IF, WB) LS‑C748993

Polyclonal Rabbit anti‑Human FCGR3B / CD16B Antibody (IF, WB) LS‑C748993

Antibody:
FCGR3B / CD16B Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C748993-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FCGR3B / CD16B Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD16B antibody LS-C748993 is an unconjugated rabbit polyclonal antibody to CD16B (FCGR3B) from human. It is reactive with human and rat. Validated for IF and WB.
Target
Human FCGR3B / CD16B
Synonyms
FCGR3B | CD16b | CD16b antigen | CD16 | Fc-gamma RIIIb | FCR-10 | FCRIII | Fc-gamma RIII | Fc-gamma RIII-beta | FCG3 | IGFR3 | Fc-gamma receptor IIIb (CD 16) | FCGR3 | FCRIIIb | Igg fc receptor iii-1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human FCGR3B (NP_000561.3). MWQLLLPTALLLLVSAGMRTEDLPKAVVFLEPQWYSVLEKDSVTLKCQGAYSPEDNSTQWFHNENLISSQASSYFIDAATVNDSGEYRCQTNLSTLSDPVQLEVHIGWLLLQAPRWVFKEEDPIHLRCHSWKNTALHKVTYLQNGKDRKYFHHNSDFHIPKATLKDSGSYFCRGLVGSKNVSSETVNITITQGLAVSTISSFSPPGYQVSFCLVMVLLFAVDTGLYFSVKTNI
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 26kDa, while the observed MW by Western blot was 50kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FCGR3B / CD16B
O75015 NM_000570 NP_000561.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024